{"id":8389,"date":"2023-04-24T14:59:19","date_gmt":"2023-04-24T18:59:19","guid":{"rendered":"https:\/\/nnyagdev.org\/?page_id=8389"},"modified":"2023-04-25T10:15:20","modified_gmt":"2023-04-25T14:15:20","slug":"persistent-biocontrol-nematode-protocols-development-timeline","status":"publish","type":"page","link":"https:\/\/nnyagdev.org\/index.php\/persistent-biocontrol-nematode-protocols-development-timeline\/","title":{"rendered":"Persistent Biocontrol Nematode Protocols Development Timeline"},"content":{"rendered":"<p><strong><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-8420 size-thumbnail\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/asbwebcovercollagesized-150x135.png\" alt=\"Photos of alfalfa snout beetle related field trials\" width=\"150\" height=\"135\" srcset=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/asbwebcovercollagesized-150x135.png 150w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/asbwebcovercollagesized-300x271.png 300w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/asbwebcovercollagesized.png 680w\" sizes=\"auto, (max-width: 150px) 100vw, 150px\" \/>PERSISTENT BIOCONTROL NEMATODE PROTOCOLS DEVELOPMENT TIMELINE<\/strong><\/p>\n<p>The use of persistent biocontrol nematodes native to northern New York State as a pest management solution begins with an invasive pest, a dairy farmer asking for help, and the commitment of his fellow farmers for finding a solution to alfalfa snout beetle. What no one anticipated was just how far pioneering the science to develop that solution would reach&#8230;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-4275 size-thumbnail\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2015\/03\/GotASB722.5-150x118.jpg\" alt=\"Alfalfa snout beetle\" width=\"150\" height=\"118\" \/><\/p>\n<p><strong>May 4, 1896:<\/strong>\u00a0 <strong>Alfalfa Snout Beetle (ASB) arrives in New York State<\/strong><br \/>\nAlfalfa snout beetle (ASB) was first detected in New York State on May 4, 1896. ASB was introduced to the port of Oswego through shipping ballast from Europe. Once alfalfa was introduced as a forage crop, ASB would soon become the most destructive alfalfa crop pest wherever ASB was found.<\/p>\n<p>The timeline that follows moves from the 2023 NNYADP persistent biocontrol project milestones back through time to the arrival of ASB in New York State. This chronicle traces the pioneering development &#8211; through laboratory and field trials on northern NY farms &#8211; of the science that would become a nature-based biocontrol not only for ASB but for other field crop and berry pests, and potentially vegetable and hops crops. All this was made possible by the long term commitment of the Northern New York Agricultural Development Program and the New York State Legislature&#8230; and the success story it is still being written today as field application and research trials continue throughout the U.S. with funding by other organizations and farmers themselves.<\/p>\n<p>See additional information and resources links at the end of this timeline.<\/p>\n<figure id=\"attachment_8391\" aria-describedby=\"caption-attachment-8391\" style=\"width: 300px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-8391\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-300x235.jpg\" alt=\"New York State map showing areas with alfalfa snout beetle\" width=\"300\" height=\"235\" srcset=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-300x235.jpg 300w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-1024x803.jpg 1024w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-150x118.jpg 150w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-768x602.jpg 768w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map-1536x1205.jpg 1536w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/nys-map.jpg 1553w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-8391\" class=\"wp-caption-text\"><em>Areas with known alfalfa snout beetle infestation.<\/em><\/figcaption><\/figure>\n<p><strong>2023:\u00a0<\/strong> An estimated 45,000 acres of farmland in northern NY have received persistent biocontrol nematodes for pest management.<\/p>\n<p><strong>2022-2023:\u00a0 <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/NNYADP2022BiocontrolReportFINAL.pdf\">A Farmer-\/Applicator-Friendly Persistent Biocontrol Nematodes Formulation for Field Application report<\/a><\/span><\/strong><\/p>\n<p>In 2022, with funding from multiple sources and by farmers themselves, persistent biocontrol nematodes were applied in New York, Idaho, Illinois, Iowa, Nebraska, Wisconsin, and eastern Colorado, primarily for management of corn rootworm, but also as a trial for managing wireworm in hops crops.<\/p>\n<p><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/NNYADP2022BiocontrolReportFINAL.pdf\"><strong><span style=\"color: #0000ff;\">2021:\u00a0 <\/span><\/strong><\/a><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2022\/02\/NNYADP22ASBResistReportFINALwebres.pdf\"><strong>Breeding Alfalfa Cultivars with Higher Resistance to ASB<\/strong><\/a><\/span><br \/>\n<a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2022\/02\/NNYADP22ASBResistReportFINALwebres.pdf\">NNYADP Project Report:\u00a0 On-Farm Selection of Surviving Alfalfa Plants<\/a><\/p>\n<p><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/NNYADP2022BiocontrolReportFINAL.pdf\"><strong><span style=\"color: #0000ff;\">August 2021: Biocontrol Nematodes Requested by NNY Potato Grower<\/span><\/strong><\/a><br \/>\n<a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/NNYADP2022BiocontrolReportFINAL.pdf\">A Northern New York\/Franklin County potato grower requests a custom application of persistent biocontrol nematodes for wireworm management.<\/a><\/p>\n<p><strong>2020: Extension, Researchers, Growers in Other State Take Note of NNYADP Project Success<\/strong><br \/>\nTexas A&amp;M AgriLife Extension posted a <a href=\"https:\/\/www.youtube.com\/watch?v=gOv9uS8Xewo\"><em>\u201cResults of Entomopathogenic Nematode Studies for Control of Corn Rootworm 2017-2019\u201d<\/em><\/a> video (20:51 min), produced by Texas A&amp;M&#8217;s Patrick Porter and Ed Bynum, and Katelyn Kesheimer of Auburn University, Alabama, highlighting how the science of biocontrol nematode use developed in Northern New York was tested in 2017-2019 to help corn growers in Texas and New Mexico. This research began testing and proving the opportunity to apply the biocontrol nematodes by center pivot irrigation system.<\/p>\n<p><strong>2020 NNYADP Report:<\/strong> <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2020\/04\/NNYADP20BiocontrolNematodeReportFINAL.pdf\">Evaluation of\u00a0 Alternative Application Methods of Biocontrol Nematodes in Alfalfa and Corn<\/a><\/span><\/p>\n<p><strong>2019: Biocontrol Nematodes Application Alternative<\/strong><br \/>\nNNYADP Project Report: <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2020\/04\/NNYADP20BiocontrolNematodeReportFINAL.pdf\">Biocontrol Nematodes Application Alternative: Mixed with Liquid Manure Applied to Corn and Alfalfa Fields<\/a><\/span> &#8211; CCE Jefferson-initiated project<\/p>\n<p><strong>2019: Grow NY Finalist Establishes Persistent Biocontrol Nematodes Supply Enterprise<\/strong><br \/>\nThis new enterprise, a 2021 Grow NY competition finalist, is based in Austin, Texas.<\/p>\n<p><strong>2018: Three NNYADP Projects<br \/>\n<\/strong>NNYADP Project Report: <span style=\"color: #0000ff;\">Impact of Biocontrol Nematodes on Corn Rootworm During Corn Rotation: 2018<\/span><br \/>\nNNYADP Project Report:<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2019\/05\/NNYADP19NematodeApplyReportFINAL.pdf\"> Evaluating Alternative Application Methods of Biocontrol Nematodes in Alfalfa and Corn<\/a><\/span><br \/>\nNNYADP Project Report: <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2019\/05\/NNYADP19ASBResistFinal-1.pdf\">Breeding Alfalfa Varieties with Higher Resistance to ASB<\/a><\/span><\/p>\n<p><strong>2017: Impact on Corn Rootworm, Alfalfa Variety Resistance Breeding Progresses<\/strong><br \/>\nNNYADP Project Report:<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2018\/04\/NNYADP2018ReportCRWwo.pdf\"> Impact of Biocontrol Nematodes on Corn Rootworm During Corn Rotation: 2017 Trials<\/a><\/span><\/p>\n<p>NNYADP Project Report: <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2018\/04\/NNYADP18ReportASBResistwo.pdf\">Breeding for Alfalfa Varieties with Resistance to Snout Beetle: 2017<\/a><\/span><\/p>\n<figure id=\"attachment_6150\" aria-describedby=\"caption-attachment-6150\" style=\"width: 300px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-6150\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2018\/01\/CRWStephenAusmusARS1.5-300x211.jpg\" alt=\"Corn rootworm on leaf.\" width=\"300\" height=\"211\" srcset=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2018\/01\/CRWStephenAusmusARS1.5-300x211.jpg 300w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2018\/01\/CRWStephenAusmusARS1.5.jpg 450w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-6150\" class=\"wp-caption-text\">Corn rootworm; USDA\/Stephen Ausmus<\/figcaption><\/figure>\n<p><strong>2016: Impact on Corn Rootworm Develops, Focus on Farmer Adoption<br \/>\n<\/strong>NNYADP Project Report: <a href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2017\/05\/NNYADP16ASBCRWResults.pdf\"><span style=\"color: #0000ff;\">Impact of Biocontrol Nematodes on Corn Rootworm During Corn Rotation: 2016 Trials<\/span><\/a><strong><br \/>\n<\/strong><\/p>\n<p>NNYADP Project Report: <span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2017\/05\/NNYADP16ASBBioAdoptResults.pdf\">Biological Control of Alfalfa Snout Beetle: Promoting New Farmer Adoption<\/a><\/span><br \/>\nThe Shields Lab continues to offer farms interested in applying the biocontrol nematodes the option to purchase biocontrol nematodes or assistance with rearing their own nematodes on-farm with their own labor.<\/p>\n<p>NNYADP Project Report: <span style=\"color: #000080;\"><a style=\"color: #000080;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2017\/05\/NNYADP16ASBResistReporta.pdf\"><span style=\"color: #0000ff;\">Breeding Alfalfa Varieties with Resistance to Alfalfa Snout Beetle: 2016<\/span><\/a><\/span><br \/>\nCornell plant breeders continue efforts to increase the resistance levels to ASB larval root feeding so alfalfa stands have a measure of durability against significant beetle populations.<\/p>\n<p><strong>2016:\u00a0 Biocontrol Nematode Rearing Lab Established in NNY<br \/>\n<\/strong>Click here for a Spectrum TV story on how the research and technical assistance through the NNYADP-funded grants projects helped a young woman establish a new enterprise on her family farm in Moira, NY: <a href=\"https:\/\/spectrumlocalnews.com\/north-country-blog\/2016\/12\/16\/research-helps-young-nny-ag-entrepreneur-build-new-business\">https:\/\/spectrumlocalnews.com\/north-country-blog\/2016\/12\/16\/research-helps-young-nny-ag-entrepreneur-build-new-business<\/a><\/p>\n<p><strong>2015: Extending Biological Control of ASB<\/strong><br \/>\nA cost-sharing program for NNY farms to promote farmer adoption of the use of the persistent biocontrol nematodes was funded by a grant from the Northern New York Agricultural Development Program. This cost-sharing program was open to new and current participating producers, with preference given to producers with no previous nematode-treated fields. \u00a0The program allowed interested new farms the opportunity to purchase the biocontrol nematodes at a 50% discount while returning farms received a 25% discount. The program also encouraged commercial applicators to participate by applying biocontrol nematodes as a possible addition to their business structure. To date, 77 farms have applied biological control nematodes on more than 250 fields covering 12,000-14,000 acres in six NNY counties.<\/p>\n<p>NNYADP Project Report: <span style=\"color: #0000ff;\"><a href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2016\/06\/NNYADP15ViandsASBReportWeb.pdf\"><span style=\"color: #0000ff;\"><strong>Breeding ASB-Resistant Alfalfa Varieties<\/strong><\/span><\/a><strong><br \/>\n<a style=\"color: #0000ff;\" href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2016\/06\/NNYADP15ViandsASBReportWeb.2.pdf\">. . . Appendix A: Tables 1-2 with 1st and 2nd-year production results<\/a><\/strong><br \/>\n<\/span><b><a href=\"https:\/\/www.nnyagdev.org\/wp-content\/uploads\/2016\/06\/NNYADP15ASBReportweb.pdf\"><span style=\"color: #0000ff;\">\u2022 Extending Biological Control of ASB<\/span><\/a><\/b><\/p>\n<p><strong>2013-2014:<\/strong> Trials on a farm in northeastern NY showed the biocontrol nematodes effectively reduced populations of black vine weevil and strawberry root weevil (both closely related to ASB and difficult to control with conventional pesticides). The weevils had been causing as much as $30,000 in annual losses.<\/p>\n<figure id=\"attachment_4184\" aria-describedby=\"caption-attachment-4184\" style=\"width: 98px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4184 size-full\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2015\/02\/CFShieldsASBplantDunn721.5.jpg\" alt=\"Man holding an alfalfa plant with healthy root.\" width=\"98\" height=\"108\" \/><figcaption id=\"caption-attachment-4184\" class=\"wp-caption-text\"><em>Entomologist Elson Shields holds an alfalfa plant with a healthy root at NNYADP field day at ASB-resistant alfalfa breeding trial host farm in Jefferson County, NY. Photo: NNYADP<\/em><\/figcaption><\/figure>\n<p><strong>2012-2015: \u00a0First ASB-Resistant Alfalfa Seed Commercially Available<br \/>\n<\/strong>An additional 85,000 seedlings were screened for ASB larval resistance in the greenhouse; promising plants intercrossed; and promising experimental lines tested in the field against ASB populations. Field trials planted using resistant varieties on farms near Adams, NY, and Lowville, NY, were evaluated through the first two years of production.<\/p>\n<p>Alfalfa populations were selected for resistance to ASB for 7-9 cycles to determine differences in yield and root feeding damage. As a result, Seedway 9558 SBR was first made available to producers in 2013.<\/p>\n<p>The Shields Lab at Cornell University makes a unique combination of two NY-native persistent nematodes available for application on grower fields to reduce ASB populations. Growers are now able to purchase ready-to-apply nematodes to their own fields, and ahave the option to purchase starter cups and rear their own nematodes to apply to their own fields.<\/p>\n<p>Depending on the type of application method, the nematodes require 2-4 years for full effectiveness.<\/p>\n<p><strong>2008: First Field Trial of Alfalfa Varieties Bred for ASB-Resistance<\/strong><br \/>\nAfter screening more than 150,000 seedlings for ASB resistance with the backcrossing of survivors, the first field trial to evaluate resistant varieties was planted in 2008 with encouraging results.\u00a0 Subsequent trials have been planted annually on ASB-infested land since 2008.<\/p>\n<p><strong>2004-2012:\u00a0 Developing Farmer-Friendly Biocontrol Nematode Protocols<\/strong><br \/>\nResearch focus becomes developing a \u201cfarmer-friendly\u201d nematode rearing and application technique.\u00a0 Extension efforts were refocused on working with farmers and agribusiness consultants, teaching them the new techniques, and assisting them with nematode rearing and applications.\u00a0 The insect-attacking nematodes were applied\/inoculated into more than 150 ASB-infested fields in northern New York\u2019s 6 counties.<\/p>\n<p><strong>2002:<\/strong> <strong>The First Indication of Success<\/strong><br \/>\nA farmwide population of ASB crash is recorded for the first time at the farm that initiated the research request.\u00a0 It became apparent that the entomopathogenic nematodes applied there had moved throughout the farm from test plots and were responsible for the ASB population crash.<\/p>\n<p><strong>1995-2002:\u00a0<\/strong>Biological control efforts with entomopathogenic nematodes continued with field evaluations for persistence, development of application techniques using commercial sprayers, timing of application, and dose rates.<\/p>\n<figure id=\"attachment_8031\" aria-describedby=\"caption-attachment-8031\" style=\"width: 140px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"size-thumbnail wp-image-8031\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2022\/01\/IMG_1206GraceTrays1002-140x150.jpg\" alt=\"Trays of young plants.\" width=\"140\" height=\"150\" srcset=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2022\/01\/IMG_1206GraceTrays1002-140x150.jpg 140w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2022\/01\/IMG_1206GraceTrays1002.jpg 186w\" sizes=\"auto, (max-width: 140px) 100vw, 140px\" \/><figcaption id=\"caption-attachment-8031\" class=\"wp-caption-text\"><em>Plants from the NNYADP ASB-resistance trials in a Cornell University greenhouse. Photo: Julie L. Hansen<\/em><\/figcaption><\/figure>\n<p><strong>1996-1997:\u00a0 ASB-Resistant Plant Breeding Begins<br \/>\n<\/strong>A breeding program was initiated by Cornell University to develop potential alfalfa varieties that would be either more tolerant of or resistant to ASB feeding. This would require 10-20 years of intensive successive plant selection and breeding and field testing research.<\/p>\n<p><strong>1988-1989:<\/strong>Potential insecticides were evaluated as a stop-gap management option, but did not prove to be an effective management tool.<\/p>\n<p><strong>1976-1986:<\/strong> <strong>ASB Populations Explode in NY<\/strong><br \/>\nASB populations began to explode over a large area (9 NY counties). It was not uncommon for 2 million beetles per acre to exist. ASB can destroy entire fields of alfalfa in one growing season.<\/p>\n<p><strong>1939-1972:\u00a0 Chemical Treatments Banned<\/strong><br \/>\nPopulations of ASB were managed using poison baiting methods and heptachlor. In 1972, these methods were banned due to concern of environmental contamination.<\/p>\n<p><strong>1933: ASB was discovered as a pest in multiple fields in NY.<\/strong><\/p>\n<figure id=\"attachment_8421\" aria-describedby=\"caption-attachment-8421\" style=\"width: 150px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"size-thumbnail wp-image-8421\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/winter-kill-150x60.jpg\" alt=\"Alfalfa field with barren patches that resemble winterkill but are alfalfa snout beetle damage.\" width=\"150\" height=\"60\" srcset=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/winter-kill-150x60.jpg 150w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/winter-kill-300x120.jpg 300w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/winter-kill-768x307.jpg 768w, https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/winter-kill.jpg 871w\" sizes=\"auto, (max-width: 150px) 100vw, 150px\" \/><figcaption id=\"caption-attachment-8421\" class=\"wp-caption-text\"><em>ASB previous year feeding damage often looks like winter kill following spring<\/em><\/figcaption><\/figure>\n<p><strong>1920s: <\/strong>Alfalfa was introduced as a new forage crop for the dairy industry, and ASB became a significant pest almost immediately. It would increase to infest more than 500,000 acres in 9 NY counties.<\/p>\n<p><strong>May 4, 1896:<\/strong>\u00a0 <strong>Alfalfa Snout Beetle (ASB) arrives in New York State<\/strong><br \/>\nAlfalfa snout beetle (ASB) was first detected in New York State on May 4, 1896. ASB was introduced to the port of Oswego through shipping ballast from Europe.<br \/>\n<span style=\"color: #000000;\"><br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-5596 alignleft\" src=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2017\/01\/cropped-cropped-nnyag-2017-logo-rec-300x150.png\" alt=\"NNYADP logo\" width=\"300\" height=\"150\" \/>Funding for the Northern New York Agricultural Development Program is supported by the New York State Legislature through the New York State Assembly and administered by the New York State Department of Agriculture and Markets.<\/span><\/p>\n<p><strong>Click on the links below to learn more (2023):<\/strong><\/p>\n<p><strong><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/index.php\/persistent-biocontrol-nematodes-for-pest-management\/\">Persistent Bicontrol Nematodes for Pest Management<\/a><\/span><br \/>\n<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/index.php\/biocontrol-nematode-application-techniques\/\">Persistent Biocontrol Nematode Application Techniques<\/a><\/span><br \/>\n<\/strong><strong><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/index.php\/persistent-biocontrol-nematodes-instructional-manuals-and-videos\/\">Persistent Biocontrol Nematodes: Instructional Manuals &amp; Videos<\/a><\/span><br \/>\n<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/index.php\/alfalfa-snout-beetle-life-cycle-management\/\">Alfalfa Snout Beetle Life Cycle and Management<\/a><\/span><br \/>\n<span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/nnyagdev.org\/index.php\/field-crops\/research-projects\/research-alfalfa-snout-beetle\/\">NNYADP Persistent Biocontrol Nematodes Year-to-Year Reports<\/a><\/span><br \/>\n<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PERSISTENT BIOCONTROL NEMATODE PROTOCOLS DEVELOPMENT TIMELINE The use of persistent biocontrol nematodes native to northern New York State as a pest management solution begins with an invasive pest, a dairy farmer asking for help, and the commitment of his fellow farmers for finding a solution to alfalfa snout beetle. What no one anticipated was just [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"class_list":{"0":"post-8389","1":"page","2":"type-page","3":"status-publish","5":"entry"},"_links":{"self":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8389","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/comments?post=8389"}],"version-history":[{"count":22,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8389\/revisions"}],"predecessor-version":[{"id":8492,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8389\/revisions\/8492"}],"wp:attachment":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/media?parent=8389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}