{"id":8395,"date":"2023-04-24T09:14:45","date_gmt":"2023-04-24T13:14:45","guid":{"rendered":"https:\/\/nnyagdev.org\/?page_id=8395"},"modified":"2023-04-24T15:01:02","modified_gmt":"2023-04-24T19:01:02","slug":"persistent-biocontrol-nematodes-instructional-manuals-and-videos","status":"publish","type":"page","link":"https:\/\/nnyagdev.org\/index.php\/persistent-biocontrol-nematodes-instructional-manuals-and-videos\/","title":{"rendered":"Persistent Biocontrol Nematodes Instructional Manuals and Videos"},"content":{"rendered":"<p><strong>How to Rear Your Own Nematodes On-Farm <\/strong>(waxworm cups method)<strong> Instructional Resources<br \/>\n<\/strong>Please reference the instructional manual and videos below on how to rear your own nematodes for application on your farm. Before you begin, you will need to look at the required materials list and rearing timeline\u00a0 in the manual).<\/p>\n<ul>\n<li><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/ASBmanual.pdf\">Rearing and Applying Biocontrol Nematodes Manual, 2016<\/a> (PDF)<\/li>\n<li><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/SprayGuide.pdf\">Sprayer Requirement Manual<\/a><\/li>\n<li><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/RearingNematodesBrochure.pdf\">Rearing Nematodes (waxworm cup method)<\/a> (Brochure PDF)<\/li>\n<li>For wax moth larvae and other supplies (see instructional videos), contact your local Cornell Cooperative Extension office for assistance identifying current suppliers<\/li>\n<\/ul>\n<p><strong>VIDEO SERIES:<br \/>\nHow To Rear and Apply Your Own Biocontrol Nematodes<br \/>\n<em>produced by Cornell Cooperative Extension and the Shields Lab, 2012<\/em><br \/>\n<\/strong>Note: these videos highlight the initially developed waxworm cup method for rearing the persistent biocontrol nematodes with a variety of field applications. It is advisable to contact your Cornell Cooperative Extension office for assistance and instruction before beginning. For those who do not wish to raise their own nematodes, an NNYADP persistent biocontrol nematode project-trained supplier and custom applicator is based in northern NY. Ask your Cornell Cooperative Extension for additional assistance.<\/p>\n<p><strong>Life Cycle of Entomopathogenic Nematodes: How this biocontrol works in the soil (1:20 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/JV29USkY-ok?rel=0\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Overview of How to Rear and Apply Your Own Entomopathogenic Nematodes: waxworm cup method (2:14 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/AdBDmEAdO3c\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Calculating Number of Nematodes Needed for Preparation for Your Field Release (4:38 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/tZXZtNFky5U\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Calculating Cups\/Field, Solution Dilution, Coverage Rate&#8230; (3:18 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" title=\"YouTube video player\" src=\"http:\/\/www.youtube.com\/embed\/kLwCDj76za0?rel=0\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Timeline, Materials &amp; Supplies Needed for Rearing Nematodes &amp; Application(11:49 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/aD0eqbCfYAQ\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Where to Find Supplies for On-Farm Cup Method Rearing of Biocontrol Nematodes and Preparation for Field Application (4:29 minutes)<\/strong><\/p>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/QNpNjFImrN0\" width=\"853\" height=\"480\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><span style=\"display: inline-block; width: 0px; overflow: hidden; line-height: 0;\" data-mce-type=\"bookmark\" class=\"mce_SELRES_start\">\ufeff<\/span><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><strong>ASB Publications &amp; Book Chapters: <\/strong>partial list of work published through 2016<\/p>\n<p><a href=\"https:\/\/nnyagdev.org\/wp-content\/uploads\/2023\/04\/Biological-Control-of-Alfalfa-Snout-Beetle-1.pdf\">Biological Control of Alfalfa Snout Beetle with Persistent Entomopathogenic Nematodes: Expanding a Single Farm\u2019s Success to an Area-Wide Biological Control Program<\/a> (PDF)<\/p>\n<p>Testa, A. M. and J. Shields. 2016.\u00a0 Low Labor \u201cIn Vivo\u201d Mass Rearing Method for Entomopathogenic Nematodes.\u00a0 Biocontrol. (submitted)<\/p>\n<p>Taylor, R.A.J., and J. Shields. 2016.\u00a0 Revisiting Potato Leafhopper, Empoasca fabae, Migration:\u00a0 Implications in a world where invasive insects are all too common.\u00a0 American Entomologist.\u00a0 (submitted)<\/p>\n<p>Smith, E. A., J. Shields and B. A. Nault. 2016.\u00a0 Impact of adjacent habitat on colonization of onion fields by adult onion thrips (Thysanoptera:\u00a0 Thripidae).\u00a0 J. Econ Entomol.\u00a0 (submitted)<\/p>\n<p>Smith, E. A., J. Shields and B. A. Nault. 2016.\u00a0 Impact of abiotic factors on onion thrips (Thysanoptera:\u00a0 Thripidae) dispersal activity in an onion ecosystem.\u00a0 Environ. Entomol.\u00a0 (submitted)<\/p>\n<p>Smith, E. A., M. Fuchs, J. Shields and B. A. Nault. 2015.\u00a0 Long-distance dispersal potential for onion thrips (Thysanoptera:\u00a0 Thripidae) and Iris yellow spot virus (Bunyaviridae: Tospovirus) in an onion ecosystem.\u00a0 Environ. Entomol. 44:\u00a0 921-930.<\/p>\n<p>Keller, M. D. and J. Shields. 2014.\u00a0 Aerobiological sampling efficiency of media-containing Petri plates for use in lower atmosphere spore collection.\u00a0 Aerobiologia 30 :\u00a0 103-109 (Published online 6\/18\/13)<\/p>\n<p>Keller, M. D. G. C. Bergstrom and J. Shields. 2014.\u00a0 The Aerobiology of Fusarium graminearum.\u00a0 Aerobiologia. 30: 123-136.\u00a0\u00a0 (Published online 11\/5\/13)<\/p>\n<p>Goulet, E. J., A. Ditommaso and J. Shields. 2014.\u00a0 Comparative study of Mecinus janthinus (Coleoptera: Curculionidae) attack and simulated mowing for control of Linaria dalmatica (Scrophulariaceae).\u00a0 The Great Lakes Entomologist 47:\u00a0 12-22.<\/p>\n<p>Nansen, C, B. Stokes, J. James, P. Porter, J. Shields, T.Wheeler, W. G. Meikle. 2013.\u00a0 Biological control agent of larger black flour beetles \u2013 a nuisance pest developing in cotton gin trash piles.\u00a0 J. Econ. Entomol.\u00a0 106: 648-652.<\/p>\n<p>Petzold-Maxwell, J. L. , A. P. Alves , R. E. Estes , E. Gray, L. J. Meinke, E. J. Shields, S. D. Thompson, N. A. Tinsley and A. J. Gassmann. 2013.\u00a0 Applying an integrated refuge to manage western corn rootworm (Coleoptera: Chrysomelidae):\u00a0 Effects on survival, fitness and selection pressure. J. Econ. Entomol. 106: 2195-2207.<\/p>\n<p>Goulet, E. J., J. Thaler, A. Ditammaso, M. Schwarzlander, J. Shields. 2013.\u00a0 Impact of Mecinus janthinus (Coleoptera: Curculionidae) on the growth and reproduction of Linaria dalmatica (Scrophulariaceae).\u00a0 The Great Lakes Entomologist 46:\u00a0 90-98.<\/p>\n<p>Neumann, G. &amp; J. Shields. 2011.\u00a0 Long term field persistence and coexistence in three entomopathogenic nematodes, Steinernema carpocapsae Weiser, S. feltiae Filipjev, and Heterorhabditis bacteriophora Poinar.\u00a0 The Great Lakes Entomologist 44:\u00a0 42-52.<\/p>\n<p>Shields, E. J., and A. M. Testa. \u00a0 Effects of Low Temperature Storage on Fecundity and Adult Mortality for the Alfalfa Snout Beetle, Otiorhynchus ligustici (L.) (Coleoptera: Curculionidae).\u00a0 The Great Lakes Entomologist 44: 21-31.<\/p>\n<p>Aylor, D. E, D G. Schmale III, J. Shields, M. Newcomb and C. J. Nappo. 2011.\u00a0 Tracking the potato late blight pathogen in the atmosphere using unmanned aerial vehicles and Lagrangian modeling.\u00a0 Agric. For. Meteorol.\u00a0 151: 251-260.<\/p>\n<p>Sappington, T. W., K. R. Ostlie, C. DiFonzo, B. E. Hibbard, C. H. Krupke, P. Porter, S. Pueppke, E. J. Shields and J. J. Tollefson.\u00a0 2010. Conducting public-sector research on commercialized transgenic seed: In search of a paradigm that works.\u00a0 GM Crops 1 (2):\u00a0 Online open access article (<a href=\"http:\/\/www.landesbioscience.com\/journals\/gmcrops\/article\/10833\">http:\/\/www.landesbioscience.com\/journals\/gmcrops\/article\/10833<\/a>)<\/p>\n<p>Shields, E. J., A. Testa, G. Neumann, K. L. Flanders and P. C. Schroeder. 2009. Biological Control of Alfalfa Snout Beetle with a multi-species application of locally-adapted persistent entomopathogenic nematodes: The first success. American Entomologist 55: 250-257.<\/p>\n<p>VanGessel. 2009. Conyza canadensis seed ascent in the lower atmosphere. Agricultural and Forest Meteorology 149: 526-534.<\/p>\n<p>Neumann, G. &amp; E. J. Shields. 2008. Multiple-species natural enemy approach for the biological control of the alfalfa snout beetle, Otiorhynchus ligustici L. (Coleoptera: Curculionidae), using entomopathogenic nematodes. J. Econ Entomol 101: 1533-1539.<\/p>\n<p>Shields, E. J., G. Neumann and A. Testa. 2007 (published in 2008). Alfalfa Snout Beetle, Otiorhynchus ligustici L. (Coleoptera: Curculionidae): Methods for egg collection and larval rearing. The Great Lakes Entomologist 40: 62-67.<\/p>\n<p>Neumann, G. &amp; E. J. Shields. 2006. Interspecific interactions among three entomopathogenic nematodes, Steinernema carpocapsae, S. feltiae, and Heterorhabditis bacteriophora, with different foraging strategies for hosts in multi-piece sand columns. Environ. Entomol. 35: 1578-1583.<\/p>\n<p><strong>Book Chapters:<\/strong><\/p>\n<ul>\n<li>Hower, A. A. and J. Shields. Weevils and Root Borers.\u00a0 In: Lamp, W.O., R. Berberet, Higley, and C. Baird (eds.). 2007. Handbook of Forage and Rangeland Insects.\u00a0 Entomological Society of America, Lanham, MD.<\/li>\n<li>Shields, E. J. and A. A. Hower.\u00a0 Rootweevils of Alfalfa.\u00a0 In:\u00a0 Lamp, W.O\u00a0 (ed).\u00a0 2012\u00a0 Alfalfa Compendium. American Phytopathological Society.<\/li>\n<li>Shields, E. J. Ch 6 \u2013 Utilizing persistent EPNs in a conservation or a more classical biological control approach.\u00a0 In: R. Campos-Herrera (ed).\u00a0 Nematode pathogenesis of insects and other pests \u2013 ecology and applied technologies for sustainable plant and crop protection, Series: Sustainability in Plant and Crop Protection, Vol. 1 (R. Campos-Herrera ed).\u00a0 Springer. 2015. Pp. 165-184.<\/li>\n<li>Shields, E. J. and A. M. Testa.\u00a0 Ch 11 \u2013 New York Case Study:\u00a0 Biological Control of ligustici with native persistent EPNs using a more classical approach.\u00a0 In: R. Campos-Herrera (ed).\u00a0 Nematode pathogenesis of insects and other pests \u2013 ecology and applied technologies for sustainable plant and crop protection, Series: Sustainability in Plant and Crop Protection, Vol. 1 (R. Campos-Herrera ed).\u00a0 Springer. 2015. pp 285-307.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>How to Rear Your Own Nematodes On-Farm (waxworm cups method) Instructional Resources Please reference the instructional manual and videos below on how to rear your own nematodes for application on your farm. Before you begin, you will need to look at the required materials list and rearing timeline\u00a0 in the manual). Rearing and Applying Biocontrol [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"class_list":{"0":"post-8395","1":"page","2":"type-page","3":"status-publish","5":"entry"},"_links":{"self":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8395","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/comments?post=8395"}],"version-history":[{"count":23,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8395\/revisions"}],"predecessor-version":[{"id":8478,"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/pages\/8395\/revisions\/8478"}],"wp:attachment":[{"href":"https:\/\/nnyagdev.org\/index.php\/wp-json\/wp\/v2\/media?parent=8395"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}